YFX and GX contributed to the gene deletion and plasmid construction. RZ helped with the bioinformatics. HL and YZX supervised the research. The work was financially supported by grants from the National Natural Science Foundation of China and The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Akaike, T. Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics.
Awano, N. Identification and functional analysis of Escherichia coli cysteine desulfhydrases. Bibli, S. A selective and sensitive method for quantification of endogenous polysulfide production in biological samples.
Redox Biol. Chen, Z. The complete pathway of thiosulfate utilization in Saccharomyces cerevisiae. Cheng, H. Biochemical and genetic characterization of PspE and GlpE, two single-domain sulfurtransferases of Escherichia coli. Open Microbiol. Cipollone, R. Common themes and variations in the rhodanese superfamily. Cooper, A. On the chemistry and biochemistry of 3-mercaptopyruvic acid, the alpha-keto acid analog of cysteine. PubMed Abstract Google Scholar. Datsenko, K.
A novel persulfide detection method reveals protein persulfide and polysulfide-reducing functions of thioredoxin and glutathione systems. Fahey, R. Occurrence of glutathione in bacteria. Google Scholar. Gelfand, D.
Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases. Giedroc, D. A new player in bacterial sulfide-inducible transcriptional regulation.
Greiner, R. Polysulfides link H2S to protein thiol oxidation. Redox Signal. Han, Q. Kynurenine aminotransferase and glutamine transaminase K of Escherichia coli : identity with aspartate aminotransferase. Dealing with the sulfur part of cysteine: four enzymatic steps degrade l-cysteine to pyruvate and thiosulfate in Arabidopsis mitochondria.
Ida, T. Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling. Iwao, O. Kasamatsu, S. Redox signaling regulated by cysteine persulfide and protein polysulfidation.
Molecules 21, e—e Kimura, H. Production and physiological effects of hydrogen sulfide. Kolluru, G. Hydrogen sulfide chemical biology: pathophysiological roles and detection. Nitric Oxide 35, 5— Kredich, N. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium.
Kumar, S. MEGA7: molecular evolutionary genetics analysis version 7. Kuo, M. Heart Circ. Libiad, M. Organization of the human mitochondrial hydrogen sulfide oxidation pathway. Cupriavidus necator H16 uses flavocytochrome c-sulfide dehydrogenase to oxidize self-produced and spiked sulfide. Mclaggan, D. Involvement of gamma-glutamyl peptides in osmoadaptation of Escherichia coli.
Mikami, Y. Thioredoxin and dihydrolipoic acid are required for 3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide. Mironov, A. Mechanism of H2S mediated protection against oxidative stress in Escherichia coli.
Nagahara, N. Is novel signal transducer sulfur oxide involved in the redox cycle of persulfide at the catalytic site cysteine in a stable reaction intermediate of mercaptopyruvate sulfurtransferase? Cytosolic mercaptopyruvate sulfurtransferase is evolutionarily related to mitochondrial rhodanese. The mercaptopyruvate pathway in cysteine catabolism: a physiologic role and related disease of the multifunctional 3-mercaptopyruvate sulfurtransferase. Nagy, P. Mechanistic chemical perspective of hydrogen sulfide signaling.
Nitric Oxide 47, S5—S6. Oguri, T. Pan, J. Persulfide reactivity in the detection of protein s-sulfhydration. ACS Chem. Park, S. High levels of intracellular cysteine promote oxidative DNA damage by driving the fenton reaction. Paul, B. H2S signalling through protein sulfhydration and beyond. Cell Biol. Riener, C. Saund, S. The chemical biology of hydropersulfides RSSH : chemical stability, reactivity and redox roles. Shatalin, K. H2S: a universal defense against antibiotics in bacteria.
Science , — Shen, J. Staphylococcus aureus sqr encodes a type II sulfide:quinone oxidoreductase and impacts reactive sulfur speciation in cells. Biochemistry 55, — Sulfite-reducing anaerobes constitute a group associated with Clostridium spp. They are found in various environmental sources, such as soil, marine sediments, decomposing vegetation, human and animal intestines, feces and infected wounds of humans and animals, surface waters, as well as in food, especially when the hygiene conditions in the preparation They are deficient.
They are deteriorating, since they produce bad smells and, very often, blacken the product when it has iron, forming a dark precipitate of iron sulfide. These microorganisms have the ability to reduce sulfites to sulfides from amino acids and sulfur compounds. These bacteria have been proposed as indicators of high-risk water pollution. The most important advantage is that their spores survive in water much longer than the organisms of the coliform group and are resistant to disinfection, to the point that they can be detected in some water samples after having received pre-disinfection, flocculation, sedimentation, filtration and terminal disinfection.
They also have value as indicators of fecal contamination of a recent nature in food. The detection of H 2 S-producing bacteria in the laboratory is mainly based on the detection of the production of hydrogen sulphide in culture. To detect the different types of bacteria, by means of isolation in culture, the source of sulfur, the indicators of sulfides and the incubation conditions of the culture are adjusted.
H 2 S production is detected when the gas comes into contact with certain metals, such as lead, iron or bismuth, and forms sulphides with these metals black. Sulfur indicators vary between different media: peptonized iron, ferrous sulfate, ferrous or ferric ammonium sulfate, ferric citrate, sodium thiosulfate, bismuth sulfite or lead acetate.
The iron salts are widely used for the detection of members of the Enterobacteriaceae family, while the procedures with lead acetate are the most sensitive for the detection of minute amounts of H 2 S in other bacteria that are not of the Enterobacteriaceae family. Recommended sample :. Conservation and shipment of the sample :. Delivery of results :. Cost of the test :.
Do not use Google Chrome Contact. Search Search Hydrogen sulfide H2S -producing bacteria sulfate reducers, sulfite reducers, sulfur reducers, and other molecules with sulfur - Qualitative and quantitative culture; Molecular identification PCR and sequencing. Hydrogen sulfide H 2 S -producing bacteria sulfate reducers, sulfite reducers, sulfur reducers, and other molecules with sulfur : Importance in the deterioration of fish and meat products - Qualitative and quantitative culture; Molecular identification PCR and sequencing Information Molecular identification of isolated colonies in culture at the species level PCR and sequencing.
Do you have any queries? Please leave me in the comments section below. I will be happy to read your comments and reply. Cancel reply. Bismuth sulfite. Deoxycholate citrate agar DCA. Kligler iron agar KIA. Triple sugar iron TSI agar. Salmonella-Shigella SS agar.
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